Julie I. Rushbrook, Ph.D. Director
Professor of Biochemistry
Basic Science Building, Room 7-107
Ph: (718) 270-2647; FAX: (718) 270-3316 julie.rushbrook@downstate.edu
Consultation. Required prior to preparation and submission of samples for analysis. Call either of the numbers above.
.
.
.
N-terminal sequencing of proteins and peptides by Edman degradation. Samples in solution or electroblotted to PVDF membrane.
.
.
.
Mass spectrometry. • Small molecule mass determination and/or structure confirmation (MS or MS/MS)
• Mass analysis (MS) of proteins or peptides in solution.
• Protein identification by nanospray liquid chromatography mass spectrometry
(LC-MS/MS) of protease-digested solution samples or SDS-PAGE bands.
• Post-translational modification determination (phosphorylation, methylation, etc) by LC/MS/MS of solution or in-gel protein digests.
.
.
.
Reversed-phase HPLC purification of proteins, peptides and small molecules.
.
.
.
.
.
.
.
.
.
.
.
.
SAMPLE REQUIREMENTS
.
.
.
Edman degradation. Minimum of 1 pmole. Samples electroblotted to PVDF should be visible by Coomassie Brilliant Blue staining. Call for procedure used to prepare samples.
.
.
.
Mass spectrometry. For solution samples, a minimum of 0.5 pmole, preferably in a small volume (~ 20ml). SDS-gel bands for in-gel digestion should be visible with Coomassie Brilliant Blue. Call for procedure used to prepare gel samples.
.
.
.
Bands on PVDF (Edman degradation) or from an SDS-gel (for in-gel digestion/mass spectral analysis) should be as concentrated as possible, i.e., maximum protein amount in as small an area as possible.
.
.
.
.
.
.
.
.
.
.
..
.
TURNAROUND TIME
.
.
.
Usually 1-3 days.
.
.
.
.
.
.
.
.
.
.
..
.
INSTRUMENTATION
.
.
.
Edman degradation: Applied Biosystems Procise Capillary LC Protein Sequencer
.
.
.
Mass spectrometry: ThermoFinnigan LCQDECA XP Ultra Sensitive Ion Trap Mass
Spectrometer
.
.
.
HPLC: Waters 626 LC System with Waters 996 Photodiode Array Detector; Applied Biosystems 130 microbore HPLC system
.
.
.
.
.
.
CHARGES
.
.
University
Industry
.
.
Consultation on any protein research problem.
Call for appointment.
No
charge
NA
.
.
Consultation prior to preparing samples for analysis - required.
No
charge
No
charge
.
.
HPLC protein/peptide separation (analytical or
preparative)/hour
$40.00
$80.00
.
.
HPLC separation run by PI’s laboratory/day
$25.00
NA
.
.
Training to run HPLC/hour
$40.00
NA
.
.
Solution-phase enzymatic digest
$25.00
$50.00
.
.
Solution-phase reduction/alkylation and enzymatic digest
$75.00
$150.00
.
.
In-gel reduction/alkylation and digestion of SDS-PAGE
protein bands
$75.00
$150.00
.
.
Electroblot transfer of protein bands from SDS-PAGE
gel to PVDF
$75.00
$150.00
.
.
Protein/peptide N-terminal sequencing
.
- Set-up + first 5 cycles
$200.00
$400.00
.
.
- Additional per cycle cost after 5 cycles
$20.00
$40.00
.
.
- Data interpretation of complex sequences
(per hour)
$40.00
$80.00
.
.
- Database search/hour
$40.00
$80.00
.
.
Mass spectrometry
.
Infusion
.
- mass analysis (ESI-MS)
$60.00
$120.00
.
.
- structure analysis (ESI-MS/MS)
$110.00
$220.00
.
.
Nanospray liquid chromatography
.
- mass analysis (LC-MS)
$100.00
$200.00
.
.
- structure analysis (LC-MS/MS)
$150.00
$300.00
.
.
Manual mass spectral data interpretation (unknowns)/hour
$40.00
$80.00
.
.
.
.
.
.
Web site design: Biomedical Communications Last updated: Tuesday, March 23, 2004
Box 8, 450 Clarkson Ave
Brooklyn, NY 11203
PROTEIN STRUCTURE LABORATORY
DEPARTMENT OF BIOCHEMISTRY
